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Bacteria-mediated tumor-targeted delivery of tumstatin (54-132) significantly suppresses tumor growth in mouse model by inhibiting angiogenesis and promoting apoptosis

《医学前沿（英文）》 2022年 第16卷 第6期   页码 873-882 doi: 10.1007/s11684-022-0925-2

摘要： Tumor growth is an angiogenesis-dependent process and accompanied by the formation of hypoxic areas. Tumstatin is a tumor-specific angiogenesis inhibitor that suppresses the proliferation and induces the apoptosis of tumorous vascular endothelial cells. VNP20009, an attenuated Salmonella typhimurium strain, preferentially accumulates in the hypoxic areas of solid tumors. In this study, a novel Salmonella-mediated targeted expression system of tumstatin (VNP-Tum5) was developed under the control of the hypoxia-induced J23100 promoter to obtain anti-tumor efficacy in mice. Treatment with VNP-Tum5 effectively suppressed tumor growth and prolonged survival in the mouse model of B16F10 melanoma. VNP-Tum5 exhibited a higher efficacy in inhibiting the proliferation and inducing the necrosis and apoptosis of B16F10 cells in vitro and in vivo compared with VNP (control). VNP-Tum5 significantly inhibited the proliferation and migration of mouse umbilical vascular endothelial cells to impede angiogenesis. VNP-Tum5 downregulated the expression of anti-vascular endothelial growth factor A, platelet endothelial cell adhesion molecule-1, phosphorylated phosphoinositide 3 kinase, and phosphorylated protein kinase B and upregulated the expression of cleaved-caspase 3 in tumor tissues. This study is the first to use tumstatin-transformed VNP20009 as a tumor-targeted system for treatment of melanoma by combining anti-tumor and anti-angiogenic effects.

关键词： Salmonella VNP20009     tumstatin     B16F10     melanoma     apoptosis     angiogenesis    

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Zfyve16 regulates the proliferation of B-lymphoid cells

Xuemei Zhao, Donghe Li, Qingsong Qiu, Bo Jiao, Ruihong Zhang, Ping Liu, Ruibao Ren

《医学前沿（英文）》 2018年 第12卷 第5期   页码 559-565 doi: 10.1007/s11684-017-0562-3

摘要：

Zfyve16 (a.k.a. endofin or endosome-associated FYVE-domain protein), a member of the FYVE-domain protein family, is involved in endosomal trafficking and in TGF-β, BMP, and EGFR signaling. The FYVE protein SARA regulates the TGF-β signaling pathway by recruiting Smad2/3 and accelerating their phosphorylation, thereby altering their susceptibility to TGF-β-mediated T cell suppression. Zfyve16 binds to Smad4 and their binding affects the formation of Smad2/3-Smad4 complex in TGF-β signaling. However, the in vivo function of Zfyve16 remains unknown. In this study, we generated a Zfyve16 knockout mouse strain (Zfyve16KO) and examined its hematopoietic phenotypes and hematopoietic reconstruction ability. The proportion of T cells in the peripheral blood of Zfyve16KO mice increases compared with that in wild-type mice. This finding is consistent with the role of Zfyve16 in facilitating TGF-β signaling. Unpredictably, B cell proliferation is inhibited in Zfyve16KO mice. The proliferation potential of Zfyve16KO B-lymphoid cells also significantly decreases in vitro. These results suggest that Zfyve16 inhibits the proliferation of T cells, possibly through the TGF-β signaling, but upregulates the proliferation of B-lymphoid cells.

关键词： Zfyve16     endofin     hematopoiesis     TGF-β     lymphocytes    

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矿井低压电网选择性漏电保护系统总体设计与实现

孙玉坤,朱志莹,黄振跃,唐平

《中国工程科学》 2012年 第14卷 第7期   页码 93-97

摘要：

设计了一款以dsPIC30F4012为主控制器、PIC16F877A为从处理器的新型主从式选择性漏电保护系统。

关键词： 漏电保护     选择性     dsPIC30F4012     PIC16F877A     RS—485总线     光纤通信    

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Effect of process parameters on the density and porosity of laser melted AlSi10Mg/SiC metal matrix composite

FAMODIMU, Mark STANFORD, Chike F. ODUOZA, Lijuan ZHANG

《机械工程前沿（英文）》 2018年 第13卷 第4期   页码 520-527 doi: 10.1007/s11465-018-0521-y

摘要：

Laser melting of aluminium alloy—AlSi10Mg has increasingly been used to create specialised products in various industrial applications, however, research on utilising laser melting of aluminium matrix composites in replacing specialised parts have been slow on the uptake. This has been attributed to the complexity of the laser melting process, metal/ceramic feedstock for the process and the reaction of the feedstock material to the laser. Thus, an understanding of the process, material microstructure and mechanical properties is important for its adoption as a manufacturing route of aluminium metal matrix composites. The effects of several parameters of the laser melting process on the mechanical blended composite were thus investigated in this research. This included single track formations of the matrix alloy and the composite alloyed with 5% and 10% respectively for their reaction to laser melting and the fabrication of density blocks to investigate the relative density and porosity over different scan speeds. The results from these experiments were utilised in determining a process window in fabricating near-fully dense parts.

关键词： selective laser melting     additive manufacturing     mechanical alloying     powder metallurgy     aluminium metal matrix composite    

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Impact analysis of compressor rotor blades of an aircraft engine

Y B SUDHIR SASTRY, B G KIROS, F HAILU, P R BUDARAPU

《结构与土木工程前沿（英文）》 2019年 第13卷 第3期   页码 505-514 doi: 10.1007/s11709-018-0493-3

摘要： Frequent failures due to foreign particle impacts are observed in compressor blades of the interceptor fighter MIG-23 aircraft engines in the Ethiopian air force, supplied by the Dejen Aviation Industry. In this paper, we made an attempt to identify the causes of failure and hence recommend the suitable materials to withstand the foreign particle impacts. Modal and stress analysis of one of the recently failed MIG-23 gas turbine compressor blades made up of the following Aluminum based alloys: 6061-T6, 7075-T6, and 2024-T4, has been performed, apart from the impact analysis of the rotor blades hit by a granite stone. The numerical results are correlated to the practical observations. Based on the modal, stress and impact analysis and the material properties of the three considered alloys, alloy 7075-T6 has been recommended as the blade material.

关键词： axial flow compressor     rotor and stator blades     aircraft engine     stress and impact analysis     aluminum alloys    

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MicroRNA-148b promotes proliferation of hair follicle cells by targeting

Wanbao YANG,Qinqun LI,Bo SU,Mei YU

《农业科学与工程前沿（英文）》 2016年 第3卷 第1期   页码 72-80 doi: 10.15302/J-FASE-2016089

摘要： MicroRNAs (miRNAs), small non-coding RNAs, are involved in many aspects of biological processes. Previous studies have indicated that miRNAs are important for hair follicle development and growth. In our study, we found by qRT-PCR that miR-148b was significantly upregulated in sheep wool follicle bulbs in anagen phase compared with the telogen phase of the hair follicle cycle. Overexpression of miR-148b promoted proliferation of both HHDPC and HHGMC. By using the TOPFlash system we demonstrated that miR-148b could activate Wnt/β-catenin pathway and , , and were consistently upregulated accordingly. Furthermore, transcript factor nuclear factor of activated T cells type 5 ( ) and were predicted to be the target of miR-148b and this was substantiated using a Dual-Luciferase reporter system. Subsequently was further identified as the target of miR-148b using western blotting. These results were considered to indicate that miR-148b could activate the Wnt/β-catenin signal pathway by targeting to promote the proliferation of human hair follicle cells.

关键词： miR-148b     hair follicle     proliferation     NFAT5     Wnt10b    

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Best operating conditions to produce hydroxyapatite nanoparticles by means of a spinning disc reactor

F. D’ Intino,B. de Caprariis,M.L. Santarelli,N. Verdone,A. Chianese

《化学科学与工程前沿（英文）》 2014年 第8卷 第2期   页码 156-160 doi: 10.1007/s11705-014-1427-9

摘要： In this work, Mg doped hydroxyapatite (Mg-HAP) nanoparticles were produced by a reaction-precipitation process by using a spinning disc reactor (SDR) at high rotational speed. The production process of these nanoparticles consisted of the neutralization reaction between two aqueous solutions of calcium chloride and ammonia orthophosphate at room temperature. By operating at pH= 10, a high purity Mg-HAP nanoparticles were obtained. In particular, they were 51 nm in average size when the two reagents were fed over the disc symmetrically at 3 cm from the disc center and a rotational speed of the disc reactor equal to 1400 r/min was adopted.

关键词： hydroxyapatite     nanoparticles     spinning disc reactor    

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Chemotactic effect of urokinase-type plasminogen activator on mouse spermatozoa

DING Xiaofang, LI Honggang, XIONG Chengliang

《医学前沿（英文）》 2008年 第2卷 第2期   页码 195-199 doi: 10.1007/s11684-008-0037-7

摘要： The aim of this study is to investigate the chemotactic effect of urokinase-type plasminogen activator (uPA) on mouse spermatozoa. Capillary assays were applied to study the chemotactic activity of ascending and descending gradients of uPA. Firstly, the chemotactic effect of an ascending gradient of uPA on mouse spermatozoa was observed by counting the number of spermatozoa that migrated into the capillary after incubation with uPA for 5, 10, 20, and 30 min, respectively, compared with that after incubation with F10. Twenty minutes was a suitable incubation time to obtain a plateau of sperm accumulation. Meanwhile, to confirm the specific effect of uPA on mouse sperm chemotaxis, uPA inhibitor (PAI-1) and anti-uPAR rabbit IgG were added to the test solution containing 20 U/mL uPA, respectively. To exclude the possibility that PAI-1 and anti-uPAR rabbit IgG may affect sperm accumulation nonspecifically, PAI-1 and anti-uPAR rabbit IgG were added to F10, respectively. It was found that the chemotactic effect of uPA was neutralized completely by PAI-1 and anti-uPAR rabbit IgG. PAI-1 and anti-uPAR rabbit IgG had no neutralizing effect on the sperm chemotactic effect. Lastly, the sperm chemotaxis response to a descending gradient of uPA was also observed. Taken together, the results suggest that uPA can induce sperm chemotaxis by binding to its receptor on the sperm membrane and may act as a chemoattractant in precontacting sperm-egg communication thereby increasing the chance encounter of spermatozoa and eggs.

关键词： chemotactic activity     receptor     uPA inhibitor     F10     chemoattractant    

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组织工程和给药技术中的三维光制造 Review

Rúben F. Pereira, Paulo J. Bártolo

《工程（英文）》 2015年 第1卷 第1期   页码 90-112 doi: 10.15302/J-ENG-2015015

摘要：

组织工程中最有前景的方法就是将生物材料、细胞和生物活性分子结合加入人工的模拟环境，用以准确地模拟人体组织修复环境，并刺激组织修复和再生。这个环境必须在细胞或亚细胞尺度上模拟出尽可能接近原生细胞外基质的主要特性，只有这样此方法才会在临床应用上有效。光制造技术通过多层工艺，如对光敏预聚物的选区光交联反应，构建包含精确结构和多相材料组合的环境。细胞和治疗分子可以包含在初始水凝胶前体的溶液中，并加工成三维（3D）结构。近来，光制造也已被开发用来动态调节水凝胶的实时特性，加强控制细胞寿命和生物活性物质的传递。本文聚焦于利用3D光制造技术为组织再生和给药技术生产先进结构的相关研究，同时介绍了目前最先进的光制造技术，重点放在控制细胞生物活性因子分布形式的工作原理和生物制造方法上。因光制造技术具有工艺快速、时空控制、高分辨率和高精度等特性，故其在复杂的3D结构设计中扮演着重要角色。这种技术同样能够为组织再生构建适当的环境，并可调节治疗方法的实施状况。

关键词： 三维光制造     生物材料     组织工程     给药    

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4-Amino-1,8-naphthalimide based fluorescent photoinduced electron transfer (PET) pH sensors as liposomal cellular imaging agents: The effect of substituent patterns on PET directional quenching

Bright, Emma B. Veale, Adam F. Henwood, D. Clive Williams, Thorfinnur Gunnlaugsson

《化学科学与工程前沿（英文）》 2020年 第14卷 第1期   页码 61-75 doi: 10.1007/s11705-019-1862-8

摘要： Four new fluorescent sensors ( - ) based on the 4-amino-1,8-naphthalimide fluorophores ( ) have been synthesized based on the classical fluorophore-spacer-receptor model. These four compounds all gave rise to emission bands centred at 535 nm, which were found to be highly pH dependent, the emission being ‘switched on’ in acidic media, while being quenched due to PET from the amino moieties to the excited state of the at more alkaline pH. The luminescent pH dependence for these probes was found to be highly dependent on the substitution on the imide site, as well as the polyamine chain attached to the position 4-amino moiety. In the case of sensor the presence of the 4-amino-aniline dominated the pH dependent quenching. Nevertheless, at higher pH, PET quenching was also found to occur from the polyamine site. Hence, is better described as a receptor -spacer -fluorophore-spacer -receptor system, where the dominant PET process is due to (normally less favourable) ‘directional’ PET quenching from the 4-amino-aniline unit to the site. Similar trends and pH fluorescence dependences were also seen for and . These compounds were also tested for their imaging potential and toxicity against HeLa cells (using DRAQ5 as nuclear stain which does now show pH dependent changes in acidic and neutral pH) and the results demonstrated that these compounds have reduced cellular viability at moderately high concentrations (with IC values between ca. 8‒30 µmol∙L ), but were found to be suitable for intracellular pH determination at 1 µmol∙L concentrations, where no real toxicity was observed. This allowed us to employ these as lysosomal probes at sub-toxic concentrations, where the based emission was found to be pH depended, mirroring that seen in aqueous solution for , with the main fluorescence changes occurring within acidic to neutral pH.

关键词： sensors     pH     photoinduced electron transfer     cellular imaging     confocal microscopy    

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Characterization and catalytic properties of mesoporous CuO/SBA-16 prepared by different impregnation

DONG Yongli, YUAN Fulong, ZHU Yujun, ZHAO Lina, CAI Zhicheng

《化学科学与工程前沿（英文）》 2008年 第2卷 第2期   页码 150-154 doi: 10.1007/s11705-008-0036-x

摘要： CuO/SBA-16 catalysts were prepared by two different routes – the conventional impregnation method and the modified impregnation method with pH adjustment. These catalysts were characterized by X-ray diffraction (XRD), atomic absorption spectrometry (AAS), N physisorption and hydrogen temperature programmed reduction (H-TPR) measurements which reveal that the cubic cage-like () pore structure of the parent SBA-16 molecule sieves was well maintained throughout the synthesis. After introduction of Cu, a different CuO dispersion exists on these catalysts. The CuO/SBA-16 prepared by modified impregnation method has a single highly dispersed CuO which is considered as a highly efficient species for hydroxylation of phenol with HO. CuO/SBA-16 prepared by the conventional impregnation method shows the presence of bulk CuO species which is undesirable for this reaction.

关键词： different     CuO/SBA-16     temperature     physisorption     introduction    

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热轧双相钢的发展现状及高强热轧双相钢的开发

袁国,利成宁,孙丹丹,康健,王国栋

《中国工程科学》 2014年 第16卷 第2期   页码 39-45

摘要：

介绍了热轧双相钢的发展现状及存在问题，指出低成本热轧双相钢、高延伸凸缘型铁素体+贝氏体热轧双相钢（F-B热轧双相钢）及高强度热轧双相钢的开发及应用，将促进我国热轧双相钢的发展，推动汽车工业的&ldquo

关键词： 热轧双相钢     低成本     F-B双相钢     析出强化     TiC     高强钢    

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医疗机构中用于控制空气传播传染病的个体患者通风隔离罩 Review

Patel, F. McGain, T. Bhatelia, S. Wang, B. Sun, J. Monty, V. Pareek

《工程（英文）》 2022年 第15卷 第8期   页码 126-132 doi: 10.1016/j.eng.2020.12.024

摘要：

作为一种局部排气通风（LEV）系统，个体患者通风（VIP）隔离罩用于将患者的空气排放物与医护人员（HCW）呼吸的空气进行分离，为减少医院获得性感染（HAI）提供了一种新的途径。最近的研究结果表明，对于典型的患者排放的气溶胶，VIP 隔离罩提供的保护与N95 口罩相当。与口罩不同的是，隔离罩的性能可以被很容易地监控，HCW可以通过警报收到故障提醒。适当使用这些相对简单的设备既可以减少感染控制对个人防护设备（PPE）的依赖，又可以为医院和诊所提供低成本、高能效的防护形式。尽管新冠病毒肺炎（COVID-19）加速了VIP 隔离罩的开发和部署，但这些设备目前仍是一项不成熟的技术。本文描述了VIP隔离罩的现状，并确定了需要进一步开发的方面，包括设备设计和相关使用规则。VIP隔离罩的概念可扩展为为个体患者提供清洁的条件和对其他环境因素如温度和湿度进行个性化控制。

关键词： 新冠病毒肺炎     个体患者通风隔离罩     空气传播     医护人员     传染病    

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Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up derivative

F. PISKUNKOVA, I. B. PAVLOVA, L. L. ZAVALOVA, I. P. BASKOVA

《化学科学与工程前沿（英文）》 2012年 第6卷 第2期   页码 203-209 doi: 10.1007/s11705-012-1277-2

摘要： Antifungal activity of recombinant medicinal leech destabilase-lysozyme (rec.Dest-Lys) was investigated by using fungi: , and , including yeasts and . Its antibacterial activity was investigated on gram-negative bacteria . These activities were assessed by radial agar diffusion assay, and scanning and transmission electron microscopy. Therefore, destabilase-lysozyme not only is endo-isopeptidase and lysozyme, but also has antifungal and antibacterial activities. Muramidase activity of rec.Dest-Lys disappeared after heat-treating at 90°C for 50 min without the loss of its antimicrobial activity. Furthermore we showed for the first time that the heated-up derivative of rec.Dest-Lys exhibited more potent activities against the above enumerated fungi and gram-negative bacteria than original protein.

关键词： recombinant destabilase-lysozyme (rec.Dest-Lys)     antimicrobial activity     antifungal activity     medicinal leech     heated-up derivative of rec.Dest-Lys (T-rec.Dest-Lys)    

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PM10 emissions from industrial coal-fired chain-grate boilers

Xinghua Li, Junzan Han, Lei Duan

《环境科学与工程前沿（英文）》 2017年 第11卷 第6期 doi: 10.1007/s11783-017-0966-y

摘要： Industrial coal-fired boiler is an important air pollutant emission source in China. The chain-grate boiler is the most extensively used type of industrial coal-fired boiler. An electrical low-pressure impactor, and a Dekati Low Pressure Impactor were applied to determine mass and number size distributions of PM at the inlet and the outlet of the particulate emission control devices at six coal-fired chain-grate boilers. The mass size distribution of PM generated from coal-fired chain-grate boilers generally displays a bimodal distribution that contains a submicron mode and a coarse mode. The PM in the submicron mode for burning with raw coal contributes to 33%±10 % of PM emissions, much higher than those for pulverized boilers. And the PM in the submicron mode for burning with briquette contributes up to 86 % of PM emissions. Multiclones and scrubbers are not efficient for controlling PM emission. Their average collection efficiencies for sub-micron particle and super-micron particle are 34% and 78%, respectively. Operating conditions of industrial steam boilers have influence on PM generation. Peak of the submicron mode during normal operation period is larger than the start-up period.

关键词： coal-fired chain-grate boiler     PM10     size distribution     particulate emission control devices     size-dependent collection efficiency    

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标题 作者 时间 类型 操作

Bacteria-mediated tumor-targeted delivery of tumstatin (54-132) significantly suppresses tumor growth in mouse model by inhibiting angiogenesis and promoting apoptosis

期刊论文

Zfyve16 regulates the proliferation of B-lymphoid cells

Xuemei Zhao, Donghe Li, Qingsong Qiu, Bo Jiao, Ruihong Zhang, Ping Liu, Ruibao Ren

期刊论文

矿井低压电网选择性漏电保护系统总体设计与实现

孙玉坤,朱志莹,黄振跃,唐平

期刊论文

Effect of process parameters on the density and porosity of laser melted AlSi10Mg/SiC metal matrix composite

FAMODIMU, Mark STANFORD, Chike F. ODUOZA, Lijuan ZHANG

期刊论文

Impact analysis of compressor rotor blades of an aircraft engine

Y B SUDHIR SASTRY, B G KIROS, F HAILU, P R BUDARAPU

期刊论文

MicroRNA-148b promotes proliferation of hair follicle cells by targeting

Wanbao YANG,Qinqun LI,Bo SU,Mei YU

期刊论文

Best operating conditions to produce hydroxyapatite nanoparticles by means of a spinning disc reactor

F. D’ Intino,B. de Caprariis,M.L. Santarelli,N. Verdone,A. Chianese

期刊论文

Chemotactic effect of urokinase-type plasminogen activator on mouse spermatozoa

DING Xiaofang, LI Honggang, XIONG Chengliang

期刊论文

组织工程和给药技术中的三维光制造

Rúben F. Pereira, Paulo J. Bártolo

期刊论文

4-Amino-1,8-naphthalimide based fluorescent photoinduced electron transfer (PET) pH sensors as liposomal cellular imaging agents: The effect of substituent patterns on PET directional quenching

Bright, Emma B. Veale, Adam F. Henwood, D. Clive Williams, Thorfinnur Gunnlaugsson

期刊论文

Characterization and catalytic properties of mesoporous CuO/SBA-16 prepared by different impregnation

DONG Yongli, YUAN Fulong, ZHU Yujun, ZHAO Lina, CAI Zhicheng

期刊论文

热轧双相钢的发展现状及高强热轧双相钢的开发

袁国,利成宁,孙丹丹,康健,王国栋

期刊论文

医疗机构中用于控制空气传播传染病的个体患者通风隔离罩

Patel, F. McGain, T. Bhatelia, S. Wang, B. Sun, J. Monty, V. Pareek

期刊论文

Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up derivative

F. PISKUNKOVA, I. B. PAVLOVA, L. L. ZAVALOVA, I. P. BASKOVA

期刊论文

PM10 emissions from industrial coal-fired chain-grate boilers

Xinghua Li, Junzan Han, Lei Duan

期刊论文